Aplikasi Bacillus sp Pada Produksi Enzim Menggunakan Metode Fermentasi Padat - Review
DOI:
https://doi.org/10.33592/unistek.v7i1.471Abstract
Solid State Fermentation (SSF) atau fermentasi padat dianggap sebagai metode fermentasi yang paling menguntungkan dari pada Submerged Fermentation (SmF) atau fermentasi terendam. Hal ini dikarenakan bahwa metode fermentasi padat (SS) lebih ramah lingkungan, produktivitas fermentasi yang lebih tinggi, konsentrasi produk dan stabilitas yang lebih tinggi, penekanan katabolik yang lebih rendah, penanaman mikroorganisme khusus untuk substrat yang tidak larut dan juga aktifitas sterilitas yang lebih rendah karena aktivitas air yang digunakan sangat rendah. Bacillus sp dapat digunakan untuk produksi enzim. Makalah ini mengulas aplikasi fermentasi padat (SSF) untuk produksi enzim yaitu amilase, esterase, xilanase, pektinase dan likenase oleh Bacillus sp. Dalam produksi enzim yang menggunakan Bacillus sp adalah Bacillus cereus yang menghasilkan enzim amilase, Bacillus sp. JB-99 yang menghasilkan enzim xilanase, Bacillus licheniformis yang menghasilkan enzim likenase, Bacillus altitudinis AP-MSU yang menghasilkan enzim esterase, Bacillus sp. DT7 menghasilkan enzim pektinase, dan banyak Bacillus sp dengan proses fermentasi padat yang menghasilkan enzim.
Â
References
Arbaciauskiene, V.S., Sruoga, A., & Butkauskas, D. (2006). Assessment of Microbial Diversity in the River Trout Salmotrutta fario L. Intestinal Tract Identified by Partial 16S rRNA Gene Sequence Analysis. Fish. Sci, 72, 597–602.
A.Pandey., P. Selvakumar., Carlos. R.S., & Poonam. N. (1999). Solis-state Fermentation for the Production of Industrial Enzyme. Fermentation-Science & Tecnology, 77(1), 149-162.
Ashis, K.M., Hemanta, A., & Sudhir, K.R. (2008). Production of Alkaline Protease By A Thermophilic Bacillus subtilis Under Solid-state Fermentation (SSF) Condition Using Imperata cylindrica Grass and Potato Peel As Low-cost Medium: Characterization and Application of Enzyme in Detergent Formulation. Biochemical Engineering Journal, 39, 353–361.
Bakir, U., Yavascaoglu, S., Guvenc, F., & Ersayin, A. Anendo-β-1,4-xylanase from Rhizopus oryzae: production, partial purification and biochemical characterization. Enz Microb Technol 2001;29:328–34.
Bornscheuer, Y.T. (2002). Microbial Carboxyl Esterases: Classification, Properties and Application in Biocatalysis. FEMS Microbiol, 26, 73–81.
Celestino, K.R.S., Cunha, R.B., & Felix, C.R. (2006). Characterization of a β-glucanase Produced by Rhizopus Microsporus var. Microsporus, and its Potential for Application in the Brewing Industry. BMC Biochem. 7, 23.
Des, R.K., Sanjeev, K.S., & Rupinder, T. (2003). Enhanced Production of Pectinase by Bacillus sp. DT7 using Solid state Fermentation. Bioresource Technology, 88, 251–254.
Fatma, C., Amel, K., Fatma, B., Monia, B., Raoudha, E., & Semia, E.C. (2012). Statistical Optimization for the Production of Lichenase by a Newly Isolated Bacillus licheniformis UEB CF in Solid state Fermentation Using Pea Pomace as a Novel Solid Support. Industrial Crops and Products, 40. 192– 198.
Gessesse, A., & Mamo, G., (1999). High Level Xylanase Production by an Alkalophilic Bacillus sp. by Using Solid state Fermentation. Enzyme Microb Technol. 25, 68–72.
Kulkarni, N., Shendye, A., & Rao, M. (1999). Molecular and Biotechnological Aspects of Xylanases. FEMS Microbial, 23, 41–456.
Palanichamy, E., Rajamony,U., Arunachalam, P., & Grasian, I. (2012). Solid-state Production of Esterase Using Fish Processing Wastes by Bacillus altitudinis AP-MSU. Food and bioproducts processing, 90, 370–376.
Ponnuswamy, V., M. Kalaiyarasi., & Samuel, G. P. V. (2015). Cow Dung is an Ideal Fermentation Medium for Amylase Production in Solid-state Fermentation by Bacillus cereus. Journal of Genetic Engineering and Biotechnology, 13, 111–117.
Prajanban, J., Thongkhib, C & Kitpreechavanich, V. (2008). Selection of High β-glucanase Produced Aspergillus Strain and Factors Affecting the Enzyme Production in Solid state Fermentation. Kasetsart J. (Nat. Sci.) 42, 294–299.
Ray, R, R., Jana, S. C., & Nanda, G. (1997). Indian J. Expt, Biol, 35, 285 -288.
R.S.Prakasham., Ch. Subba. R., & P.N. Sarma. (2006). Green Gram Husk—an Inexpensive Substrate for Alkaline Protease Production by Bacillus sp. in Solid-state Fermentation. Bioresource Technology, 97, 1449–1454.
S.Virupakshi., K. Gireesh. B., Satish. R. G., & G.R. Naik. (2005). Production of a Xylanolytic Enzyme by a Thermoalkaliphilic Bacillus sp. JB-99 in Solid state Fermentation. Process Biochemistry, 40, 431–435.
